Structure of PDB 3d4p Chain B

Receptor sequence

>3d4pB (length=310) Species: 93062 (Staphylococcus aureus subsp. aureus COL)

FKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKH
ATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIF
KSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDS
ARFRLLLSEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQ
RPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDA
VLTVSALLEGEYEEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSA
KTLKDIMAEA

3D structure

• PDB: 3d4p Crystal structure of Lactate Dehydrogenase from Staphylococcus Aureus complexed with NAD and pyruvate
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R92 D152 R155 H179
• Catalytic site (residue number reindexed from 1): R89 D149 R152 H176
• Enzyme Commision number: 1.1.1.27: L-lactate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	G15 A16 V17 D38 L39 A82 G83 A122 N124 H179 T232 V236	G12 A13 V14 D35 L36 A79 G80 A119 N121 H176 T229 V233
BS02	PYR	B	L151 R155 H179 A222 T232	L148 R152 H176 A219 T229

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006096 glycolytic process
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3d4p, PDBe:3d4p, PDBj:3d4p
• PDBsum: 3d4p
• UniProt: Q5HJD7|LDH1_STAAC L-lactate dehydrogenase 1 (Gene Name=ldh1)