Structure of PDB 3cmv Chain B

Receptor sequence

>3cmvB (length=1163) Species: 83333 (Escherichia coli K-12)

VETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQRE
GKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSG
AVDVIVVDSVAALTPKAEIEGEISHMGLAARMMSQAMRKLAGNLKQSNTL
LIFINQGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAP
FKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKAN
ATAWLKDNPETAKEIEKKVRELLLKQKALAAALGQIEKQFGKGSIMRLGE
DRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIA
AAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDA
LARSGAVDVIVVDSVAALTPKAEIGLAARMMSQAMRKLAGNLKQSNTLLI
FINQGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFK
QAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANAT
AWLKDNPETAKEIEKKVRELLLKQKALAAALGQIEKQFGKGSIMRLGEDR
SMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAA
QREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALA
RSGAVDVIVVDSVAALTPKAEIGLAARMMSQAMRKLAGNLKQSNTLLIFI
NQGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQA
EFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAW
LKDNPETAKEIEKKVRELLLKQKALAAALGQIEKQFGKGSIMRLGEDRSM
DVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQR
EGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARS
GAVDVIVVDSVAALTPLAARMMSQAMRKLAGNLKQSNTLLIFINQGGNAL
KFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYG
EGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPET
AKEIEKKVRELLL

3D structure

• PDB: 3cmv Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures.
• Chain: B
• Resolution: 4.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	T73 D144	T37 D108
BS02	MG	B	T2073 L2099	T640 L666
BS03	MG	B	T3073 D3144	T938 D1009
BS04	ANP	B	E68 S69 G71 K72 T73 T74 Y103	E32 S33 G35 K36 T37 T38 Y67
BS05	ANP	B	S1069 G1071 K1072 T1073 T1074	S338 G340 K341 T342 T343
BS06	ANP	B	E2068 S2069 G2071 K2072 T2073 T2074	E635 S636 G638 K639 T640 T641
BS07	ANP	B	E3068 S3069 G3071 K3072 T3073 T3074 Y3103 Y3264	E933 S934 G936 K937 T938 T939 Y968 Y1099

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008094 ATP-dependent activity, acting on DNA
• GO:0016887 ATP hydrolysis activity
• GO:0140297 DNA-binding transcription factor binding
• GO:0140664 ATP-dependent DNA damage sensor activity

Biological Process

• GO:0000725 recombinational repair
• GO:0006259 DNA metabolic process
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0006974 DNA damage response
• GO:0009432 SOS response
• GO:0010212 response to ionizing radiation
• GO:0019985 translesion synthesis
• GO:0035825 homologous recombination
• GO:0048870 cell motility

Cellular Component

• GO:0005737 cytoplasm
• GO:0009355 DNA polymerase V complex

External links

• PDB: RCSB:3cmv, PDBe:3cmv, PDBj:3cmv
• PDBsum: 3cmv
• PubMed: 18497818
• UniProt: P0A7G6|RECA_ECOLI Protein RecA (Gene Name=recA)