Structure of PDB 3c1n Chain B

Receptor sequence

>3c1nB (length=459) Species: 2190 (Methanocaldococcus jannaschii)

TTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVE
ISQQALDVRDIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRI
EELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIAL
EGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGY
ITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPK
LSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITND
SDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQ
GSSETNISLVVSEEDVDKALKALKREFGDLNNNLIRDVSVDKDVCVISVV
GAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCV
RKLHEKFIE

3D structure

• PDB: 3c1n The Structural Basis for Allosteric Inhibition of a Threonine-sensitive Aspartokinase.
• Chain: B
• Resolution: 2.72 Å

Enzymatic activity

• Enzyme Commision number: 2.7.2.4: aspartate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	THR	B	N434 I435	N424 I425
BS02	THR	B	A417 K418 I420 A421 Q440 E444	A407 K408 I410 A411 Q430 E434
BS03	THR	B	G8 T10 S11 W229 T230 D231	G7 T9 S10 W228 T229 D230

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004072 aspartate kinase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity

Biological Process

• GO:0008652 amino acid biosynthetic process
• GO:0009088 threonine biosynthetic process
• GO:0009089 lysine biosynthetic process via diaminopimelate
• GO:0009090 homoserine biosynthetic process
• GO:0016310 phosphorylation

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:3c1n, PDBe:3c1n, PDBj:3c1n
• PDBsum: 3c1n
• PubMed: 18334478
• UniProt: Q57991|AK_METJA Probable aspartokinase (Gene Name=MJ0571)