Structure of PDB 3bzu Chain B

Receptor sequence
>3bzuB (length=262) Species: 9606 (Homo sapiens) [Search protein sequence]
PLNEEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETL
QKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHI
TNTSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSL
AGKVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTE
TAMKAVSGIVHMQAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNP
SRKILEFLYSTS
3D structure
PDB3bzu Structural characterization and pharmacodynamic effects of an orally active 11beta-hydroxysteroid dehydrogenase type 1 inhibitor.
ChainB
Resolution2.25 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S170 Y183 K187
Catalytic site (residue number reindexed from 1) S149 Y162 K166
Enzyme Commision number 1.1.1.146: 11beta-hydroxysteroid dehydrogenase.
1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP(+)).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP B G41 S43 K44 G45 I46 R66 S67 T92 M93 N119 H120 I121 V168 S170 Y183 K187 L215 G216 L217 I218 T220 T222 A223 G20 S22 K23 G24 I25 R45 S46 T71 M72 N98 H99 I100 V147 S149 Y162 K166 L194 G195 L196 I197 T199 T201 A202
BS02 A21 B T124 L126 L171 A172 Y177 Y183 L217 T103 L105 L150 A151 Y156 Y162 L196 MOAD: ic50=14nM
BindingDB: Ki=22nM
Gene Ontology
Molecular Function
GO:0005496 steroid binding
GO:0016491 oxidoreductase activity
GO:0042803 protein homodimerization activity
GO:0047022 7-beta-hydroxysteroid dehydrogenase (NADP+) activity
GO:0050661 NADP binding
GO:0070524 11-beta-hydroxysteroid dehydrogenase (NADP+) activity
GO:0102196 cortisol dehydrogenase activity
Biological Process
GO:0006706 steroid catabolic process
GO:0008202 steroid metabolic process
GO:0030324 lung development
Cellular Component
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3bzu, PDBe:3bzu, PDBj:3bzu
PDBsum3bzu
PubMed18069989
UniProtP28845|DHI1_HUMAN 11-beta-hydroxysteroid dehydrogenase 1 (Gene Name=HSD11B1)

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