Structure of PDB 3bv0 Chain B

Receptor sequence

>3bv0B (length=414) Species: 1773 (Mycobacterium tuberculosis)

GGLTPEQIIAVDGAHLWHPSPVVAVAAHGAWLTLIRDGQPIEVLDAMSSW
WTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLLVDITPAG
LDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHGDTFLA
MSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQLAQHAGEL
AAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATGFGRTG
ALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGPTFMAN
PLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTARALPAVTDVRVCG
AIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAMPPYICTPAEITQI
TSAMVEVARLVGSL

3D structure

• PDB: 3bv0 Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
• Chain: B
• Resolution: 2.21 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y143 E206 D240 A243 K269 Y385
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	P317 T318	P295 T296
BS02	PLP	B	W64 G124 S125 Y157 H158 G159 E220 D254 I256 K283	W50 G110 S111 Y143 H144 G145 E206 D240 I242 K269

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3bv0, PDBe:3bv0, PDBj:3bv0
• PDBsum: 3bv0
• PubMed: 20565114
• UniProt: P9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)