Structure of PDB 3bam Chain B

Receptor sequence
>3bamB (length=209) Species: 1390 (Bacillus amyloliquefaciens) [Search protein sequence]
MEVEKEFITDEAKELLSKDKLIQQAYNEVKTSICSPIWPATSKTFTINNT
EKNCNGVVPIKELCYTLLEDTYNWYREKPLDILKLEKKKGGPIDVYKEFI
ENSELKRVGMEFETGNISSAHRSMNKLLLGLKHGEIDLAIILMPIKQLAY
YLTDRVTNFEELEPYFELTEGQPFIFIGFNAEAYNSNVPLIPKGSDGMSK
RSIKKWKDK
3D structure
PDB3bam The role of metals in catalysis by the restriction endonuclease BamHI.
ChainB
Resolution1.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B V57 K89 G90 G91 E111 E113 T114 S119 R122 K126 T153 D154 R155 K193 V57 K89 G90 G91 E111 E113 T114 S119 R122 K126 T153 D154 R155 K193
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:3bam, PDBe:3bam, PDBj:3bam
PDBsum3bam
PubMed9783752
UniProtP23940|T2BA_BACAM Type II restriction enzyme BamHI (Gene Name=bamHIR)

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