Structure of PDB 3b60 Chain B

Receptor sequence

>3b60B (length=572)

WQTFRRLWPTIAPFKAGLIVAGIALILNAASDTFMLSLLKPLLDDGFGKT
DRSVLLWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRRRLFGHMM
GMPVAFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIM
MFYYSWQLSIILVVLAPIVSIAIRVVSKRFRSISKNMQNTMGQVTTSAEQ
MLKGHKEVLIFGGQEVETKRFDKVSNKMRLQGMKMVSASSISDPIIQLIA
SLALAFVLYAASFPSVMDSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQ
RGMAACQTLFAILDSEQEKDEGKRVIDRATGDLEFRNVTFTYPGREVPAL
RNINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGHILMDGHDLRE
YTLASLRNQVALVSQNVHLFNDTVANNIAYARTEEYSREQIEEAARMAYA
MDFINKMDNGLDTIIGENGVLLSGGQRQRIAIARALLRDSPILILDEATS
ALDTESERAIQAALDELQKNRTSLVIAHRLSTIEQADEIVVVEDGIIVER
GTHSELLAQHGVYAQLHKMQFG

3D structure

• PDB: 3b60 Flexibility in the ABC transporter MsbA: Alternating access with a twist.
• Chain: B
• Resolution: 3.7 Å

Enzymatic activity

• Enzyme Commision number: 7.5.2.6: ABC-type lipid A-core oligosaccharide transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	B	L480 S482	L471 S473
BS02	ANP	B	Y351 S378 G379 S380 G381 K382 S383 T384	Y342 S369 G370 S371 G372 K373 S374 T375

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0034040 ATPase-coupled lipid transmembrane transporter activity
• GO:0042802 identical protein binding
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006869 lipid transport
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:3b60, PDBe:3b60, PDBj:3b60
• PDBsum: 3b60
• PubMed: 18024585
• UniProt: P63359|MSBA_SALTY ATP-dependent lipid A-core flippase (Gene Name=msbA)