Structure of PDB 3b12 Chain B

Receptor sequence
>3b12B (length=294) Species: 36773 (Burkholderia sp.) [Search protein sequence]
MFEGFERRLVDVGDVTINCVVGGSGPALLLLHGFPQNLHMWARVAPLLAN
EYTVVCADLRGYGGSSKPVGAPDHANYSFRAMASDQRELMRTLGFERFHL
VGHARGGRTGHRMALDHPDSVLSLAVLDIIPTYVMFEEVDRFVARAYWHW
YFLQQPAPYPEKVIGADPDTFYEGCLFGWGATGADGFDPEQLEEYRKQWR
DPAAIHGSCCDYRAGGTIDFELDHGDLGRQVQCPALVFSGSAGLMHSLFE
MQVVWAPRLANMRFASLPGGHFFVDRFPDDTARILREFLSDARS
3D structure
PDB3b12 Substrate specificity of fluoroacetate dehalogenase: an insight from crystallographic analysis, fluorescence spectroscopy, and theoretical computations
ChainB
Resolution1.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) F34 N37 H103 A104 R105 H149 Y212 G243 H271
Catalytic site (residue number reindexed from 1) F34 N37 H103 A104 R105 H149 Y212 G243 H271
Enzyme Commision number 3.8.1.3: haloacetate dehalogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAH B A104 R105 R108 W150 Y212 H271 A104 R105 R108 W150 Y212 H271
BS02 FAH B D223 R258 D223 R258
BS03 MG B Y77 F79 D211 D219 Y77 F79 D211 D219
Gene Ontology
Molecular Function
GO:0016787 hydrolase activity
GO:0018785 haloacetate dehalogenase activity

View graph for
Molecular Function
External links
PDB RCSB:3b12, PDBe:3b12, PDBj:3b12
PDBsum3b12
PubMed22674735
UniProtQ1JU72|DEHA_BURSP Fluoroacetate dehalogenase (Gene Name=fac-dex)

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