Structure of PDB 3ay6 Chain B

Receptor sequence

>3ay6B (length=267) Species: 1404 (Priestia megaterium)

HHHHHHMYTDLKDKVVVITGGSTGLGRAMAVRFGQEEAKVVINYYNNEEE
ALDAKKEVEEAGGQAIIVQGDVTKEEDVVNLVQTAIKEFGTLDVMINNAG
VENPVPSHELSLDNWNKVIDTNLTGAFLGSREAIKYFVENDIKGNVINMS
SVHEMIPWPLFVHYAASKGGMKLMTETLALEYAPKGIRVNNIGPGAMNTP
INAEKFADPVQRADVESMIPMGYIGKPEEVAAVAAFLASSQASYVTGITL
FADGGMTKYPSFQFGRG

3D structure

• PDB: 3ay6 Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G18 S145 Y158 K162
• Catalytic site (residue number reindexed from 1): G24 S151 Y164 K168
• Enzyme Commision number: 1.1.1.47: glucose 1-dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	E96 S145 H147 W152 Y158 N196 K199	E102 S151 H153 W158 Y164 N202 K205
BS02	NAI	B	G14 T17 G18 L19 Y39 D65 V66 N92 A93 G94 V95 M143 S145 Y158 K162 P188 G189 M191 T193 P194 I195 N196	G20 T23 G24 L25 Y45 D71 V72 N98 A99 G100 V101 M149 S151 Y164 K168 P194 G195 M197 T199 P200 I201 N202

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0042802 identical protein binding
• GO:0047934 glucose 1-dehydrogenase (NAD+) activity
• GO:0047935 glucose 1-dehydrogenase (NADP+) activity
• GO:0047936 glucose 1-dehydrogenase [NAD(P)+] activity

External links

• PDB: RCSB:3ay6, PDBe:3ay6, PDBj:3ay6
• PDBsum: 3ay6
• PubMed: 22804868
• UniProt: P39485|DHG4_PRIMG Glucose 1-dehydrogenase 4 (Gene Name=gdhIV)