Structure of PDB 3ax9 Chain B

Receptor sequence

>3ax9B (length=1218) Species: 9913 (Bos taurus)

TADELVFFVNGKKVVEKNADPETTLLAYLRRKLGLRGTKLGCGEGGCGAC
TVMLSKYDRLQDKIIHFSANACLAPICTLHHVAVTTVEGIGSTKTRLHPV
QERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTVEEIEDAFQGNLCRCT
GYRPILQGFRTFAKPKQLRFEGERVTWIQASTLKELLDLKAQHPEAKLVV
GNTEIGIEMKFKNQLFPMIICPAWIPELNAVEHGPEGISFGAACALSSVE
KTLLEAVAKLPTQKTEVFRGVLEQLRWFAGKQVKSVASLGGNIITASPIS
DLNPVFMASGTKLTIVSRGTRRTVPMDHTFFPSYRKTLLGPEEILLSIEI
PYSREDEFFSAFKQASRREDDIAKVTCGMRVLFQPGSMQVKELALCYGGM
ADRTISALKTTQKQLSKFWNEKLLQDVCAGLAEELSLSPDAPGGMIEFRR
TLTLSFFFKFYLTVLKKLGDTVGRPLPHLAAAMQASGEAVYCDDIPRYEN
ELFLRLVTSTRAHAKIKSIDVSEAQKVPGFVCFLSADDIPGSNETGLFND
ETVFAKDTVTCVGHIIGAVVADTPEHAERAAHVVKVTYEDLPAIITIEDA
IKNNSFYGSELKIEKGDLKKGFSEADNVVSGELYIGGQDHFYLETHCTIA
IPKGEEGEMELFVSTQNAMKTQSFVAKMLGVPVNRILVRVKRMGGGFGGK
ETRSTLVSVAVALAAYKTGHPVRCMLDRNEDMLITGGRHPFLARYKVGFM
KTGTIVALEVDHYSNAGNSRDLSHSIMERALFHMDNCYKIPNIRGTGRLC
KTNLSSNTAFRGFGGPQALFIAENWMSEVAVTCGLPAEEVRWKNMYKEGD
LTHFNQRLEGFSVPRCWDECLKSSQYYARKSEVDKFNKENCWKKRGLCII
PTKFGISFTVPFLNQAGALIHVYTDGSVLVSHGGTEMGQGLHTKMVQVAS
KALKIPISKIYISETSTNTVPNSSPTAASVSTDIYGQAVYEACQTILKRL
EPFKKKNPDGSWEDWVMAAYQDRVSLSTTGFYRTPNLGYSFETNSGNAFH
YFTYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQ
GLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPTEFRVSLLRDCPNKK
AIYASKAVGEPPLFLGASVFFAIKDAIRAARAQHTNNNTKELFRLDSPAT
PEKIRNACVDKFTTLCVT

3D structure

• PDB: 3ax9 Protein conformational gating of enzymatic activity in xanthine oxidoreductase
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q767 E802 R880 H884 R912 G1260 E1261
• Catalytic site (residue number reindexed from 1): Q666 E701 R779 H783 R811 G1159 E1160
• Enzyme Commision number: 1.17.1.4: xanthine dehydrogenase.
  1.17.3.2: xanthine oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	B	C113 G114 C116 C148 C150	C112 G113 C115 C147 C149
BS02	FES	B	G42 C43 G44 G46 C48 G49 C51 N71 C73	G41 C42 G43 G45 C47 G48 C50 N70 C72
BS03	FAD	B	G46 K256 L257 V259 G260 N261 T262 E263 I264 F337 V342 A346 S347 G350 N351 I353 T354 S359 D360 I403 L404	G45 K197 L198 V200 G201 N202 T203 E204 I205 F278 V283 A287 S288 G291 N292 I294 T295 S300 D301 I344 L345
BS04	XAX	B	Q112 C150 G796 G797 F798 G799 F911 R912 M1038 G1039 Q1040 A1078 A1079 S1080 S1082 Q1194 E1261	Q111 C149 G695 G696 F697 G698 F810 R811 M937 G938 Q939 A977 A978 S979 S981 Q1093 E1160
BS05	CA	B	A867 S870 R871 S874 S907 N908	A766 S769 R770 S773 S806 N807
BS06	BCT	B	R839 H840 T909 A910 G915 Q918	R738 H739 T808 A809 G814 Q817

Gene Ontology

Molecular Function

• GO:0004854 xanthine dehydrogenase activity
• GO:0004855 xanthine oxidase activity
• GO:0005506 iron ion binding
• GO:0016491 oxidoreductase activity
• GO:0030151 molybdenum ion binding
• GO:0042803 protein homodimerization activity
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0071949 FAD binding

Biological Process

• GO:0009115 xanthine catabolic process

Cellular Component

• GO:0002197 xanthine dehydrogenase complex
• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0005737 cytoplasm
• GO:0005777 peroxisome

External links

• PDB: RCSB:3ax9, PDBe:3ax9, PDBj:3ax9
• PDBsum: 3ax9
• PubMed: 22145797
• UniProt: P80457|XDH_BOVIN Xanthine dehydrogenase/oxidase (Gene Name=XDH)