Structure of PDB 3awi Chain B

Receptor sequence
>3awiB (length=628) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFR
EAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMP
LPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGFAPAK
NPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFG
RKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALL
RRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANA
VEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQN
LSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVAGQVS
HIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDD
QQQNRQRLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYE
ATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQM
SDEPIPMDASTLAALNPNRLWVRKLLKG
3D structure
PDB3awi Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli
ChainB
Resolution3.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.5.-.-
2.1.1.61: tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B I270 G271 G272 G273 I274 A275 C294 A295 D296 A303 S304 N306 A310 Q431 L432 N461 Q464 Y504 G565 G616 S617 G619 L620 I236 G237 G238 G239 I240 A241 C260 A261 D262 A269 S270 N272 A276 Q397 L398 N427 Q430 Y470 G531 G582 S583 G585 L586
Gene Ontology
Molecular Function
GO:0004808 tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
GO:0008168 methyltransferase activity
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
GO:0016491 oxidoreductase activity
GO:0016645 oxidoreductase activity, acting on the CH-NH group of donors
GO:0050660 flavin adenine dinucleotide binding
GO:0071949 FAD binding
Biological Process
GO:0002097 tRNA wobble base modification
GO:0002098 tRNA wobble uridine modification
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3awi, PDBe:3awi, PDBj:3awi
PDBsum3awi
PubMed21574198
UniProtP77182|MNMC_ECOLI tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (Gene Name=mnmC)

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