Structure of PDB 2zzg Chain B

Receptor sequence

>2zzgB (length=737) Species: 53953 (Pyrococcus horikoshii)

EFIMKTRMFEEEGWIRKKCKVCGKPFWTLDPDRETCGDPPCDEYQFIGKP
GIPRKYTLDEMREKFLRFFEKHEIYPHGRVKRYPVLPRWRDDVLLVGASI
MDFQPWVISGEADPPANPLVISQPSIRFTDIDNVGITGRHFTIFEMMAHH
AFNYPGKPIYWMDETVELAFEFFTKELKMKPEDITFKENPWAGGGNAGPA
FEVLYRGLEVATLVFMQYKKAPQDQVVVIKGEKYIPMETKVVDTGYGLER
LVWMSQGTPTAYDAVLGYVVEPLKKMAGIEKIDEKILMENSRLAGMFDIE
DLRYLREQVAKRVGITVEELEKAIRPYELIYAIADHTKALTFMLADGVVP
SNVKAGYLARLLIRKSIRHLRELGLEVPLSEIVALHIKELHKTFPEFKEM
EDIILEMIELEEKKYAETLRRGSDLVRREIAKLKKKGIKEIPVEKLVTFY
ESHGLTPEIVKEIAEKEGVKVNIPDNFYSMVAKEAERTLVDFELLKDLPD
TRRLYYEDPFMKEFDAKVLRVIKDWVILDATAFYPEGGGQPYDTGVLIVN
GREVKVTNVQKVGKVIIHKVEDPGAFKEGMIVHGKIDWKRRIQHMRHHTG
THVLMGALVRVLGRHVWQAGSQLTTDWARLDISHYKRISEEELKEIEMLA
NRIVMEDRKVTWEWLPRTTAEQKYGFRLYQGGVVPGREIRVVKIEDWDVQ
ACGGTHLPSTGLVGPIKILRTERIQDGVERIIFACGE

3D structure

• PDB: 2zzg The structure of alanyl-tRNA synthetase with editing domain.
• Chain: B
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.7: alanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C20 C23 C37 C42	C19 C22 C36 C41
BS02	ZN	B	H613 H617 C717	H598 H602 C702
BS03	A5A	B	A99 R128 R140 F142 F145 M147 W192 E210 V211 A212 T213 D248 G250 R255	A98 R127 R139 F141 F144 M146 W191 E209 V210 A211 T212 D243 G245 R250

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004813 alanine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006419 alanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2zzg, PDBe:2zzg, PDBj:2zzg
• PDBsum: 2zzg
• PubMed: 19549823
• UniProt: O58035|SYA_PYRHO Alanine--tRNA ligase (Gene Name=alaS)