Structure of PDB 2zzb Chain B

Receptor sequence

>2zzbB (length=485) Species: 9606 (Homo sapiens)

KSYDYDLIIIGGGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWGLGGT
CVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNH
IGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFL
IATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGF
LAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQ
IEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVK
INEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLY
AGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWP
LEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKC
GLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASIL

3D structure

• PDB: 2zzb Terpyridine-platinum(II) complexes are effective inhibitors of mammalian topoisomerases and human thioredoxin reductase 1.
• Chain: B
• Resolution: 3.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L55 C59 C64 K67 Y200 E204 G470 H472 E477
• Catalytic site (residue number reindexed from 1): L47 C51 C56 K59 Y192 E196 G462 H464 E469
• Enzyme Commision number: 1.11.1.2: NADPH peroxidase.
  1.8.1.9: thioredoxin-disulfide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	I18 G21 S22 D42 F43 T58 G63 C64 K67 Y131 G132 T161 Y200 R293 G333 D334 E341 L342 T343	I10 G13 S14 D34 F35 T50 G55 C56 K59 Y123 G124 T153 Y192 R285 G325 D326 E333 L334 T335
BS02	TPT	B	N107 S111 W114	N99 S103 W106

Gene Ontology

Molecular Function

• GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0045454 cell redox homeostasis

External links

• PDB: RCSB:2zzb, PDBe:2zzb, PDBj:2zzb
• PDBsum: 2zzb
• PubMed: 19525010
• UniProt: Q16881|TRXR1_HUMAN Thioredoxin reductase 1, cytoplasmic (Gene Name=TXNRD1)