Structure of PDB 2zz0 Chain B

Receptor sequence

>2zz0B (length=487) Species: 9606 (Homo sapiens)

LPKSYDYDLIIIGGGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWGLG
GTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQ
NHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAER
FLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECA
GFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKV
EQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVG
VKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQR
LYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYF
WPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAAL
KCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASIL

3D structure

• PDB: 2zz0 Terpyridine-platinum(II) complexes are effective inhibitors of mammalian topoisomerases and human thioredoxin reductase 1.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L55 C59 C64 K67 Y200 E204 G470 H472 E477
• Catalytic site (residue number reindexed from 1): L49 C53 C58 K61 Y194 E198 G464 H466 E471
• Enzyme Commision number: 1.11.1.2: NADPH peroxidase.
  1.8.1.9: thioredoxin-disulfide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	I18 G21 S22 D42 F43 G57 T58 C59 G63 C64 K67 Y131 G132 T161 Y200 V201 R293 G333 D334 E341 L342 T343	I12 G15 S16 D36 F37 G51 T52 C53 G57 C58 K61 Y125 G126 T155 Y194 V195 R287 G327 D328 E335 L336 T337

Gene Ontology

Molecular Function

• GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0045454 cell redox homeostasis

External links

• PDB: RCSB:2zz0, PDBe:2zz0, PDBj:2zz0
• PDBsum: 2zz0
• PubMed: 19525010
• UniProt: Q16881|TRXR1_HUMAN Thioredoxin reductase 1, cytoplasmic (Gene Name=TXNRD1)