Structure of PDB 2zof Chain B

Receptor sequence

>2zofB (length=478) Species: 10090 (Mus musculus)

SPNSMSALKAVFQYIDENQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMME
VAAADVQRLGGSVELVDIGKQKLPDGSEIPLPPILLGKLGSDPQKKTVCI
YGHLDVQPAALEDGWDSEPFTLVEREGKLYGRGSTDDKGPVAGWMNALEA
YQKTGQEIPVNLRFCLEGMEESGSEGLDELIFAQKDKFFKDVDYVCISDN
YWLGKNKPCITYGLRGICYFFIEVECSDKDLHSGVYGGSVHEAMTDLISL
MGCLVDKKGKILIPGINDAVAPVTDEEHALYDHIDFDMEEFAKDVGAETL
LHSCKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVP
DMIPEVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQA
GRRALKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHS
QNEKLNRLNYIEGTKMLAAYLYEVSQLK

3D structure

• PDB: 2zof Structural basis for substrate recognition and hydrolysis by mouse carnosinase CN2.
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 3.4.13.18: cytosol non-specific dipeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BES	B	H228 T330	H232 T334	MOAD: ic50=7nM PDBbind-CN: -logKd/Ki=8.15,IC50=7nM
BS02	MN	B	D132 E167 H445	D136 E171 H449
BS03	MN	B	H99 D132 D195	H103 D136 D199
BS04	BES	B	D132 E166 E167 D195 R343 E414 G416 S417 H445	D136 E170 E171 D199 R347 E418 G420 S421 H449	MOAD: ic50=7nM PDBbind-CN: -logKd/Ki=8.15,IC50=7nM

Gene Ontology

Molecular Function

• GO:0004180 carboxypeptidase activity
• GO:0008233 peptidase activity
• GO:0008237 metallopeptidase activity
• GO:0016787 hydrolase activity
• GO:0016805 dipeptidase activity
• GO:0046872 metal ion binding
• GO:0070573 metallodipeptidase activity

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:2zof, PDBe:2zof, PDBj:2zof
• PDBsum: 2zof
• PubMed: 18550540
• UniProt: Q9D1A2|CNDP2_MOUSE Cytosolic non-specific dipeptidase (Gene Name=Cndp2)