Structure of PDB 2zat Chain B

Receptor sequence

>2zatB (length=251) Species: 9823 (Sus scrofa)

KPLENKVALVTASTDGIGLAIARRLAQDGAHVVVSSRKQENVDRTVATLQ
GEGLSVTGTVCHVGKAEDRERLVAMAVNLHGGVDILVSNAAVNPFFGNII
DATEEVWDKILHVNVKATVLMTKAVVPEMEKRGGGSVLIVSSVGAYHPFP
NLGPYNVSKTALLGLTKNLAVELAPRNIRVNCLAPGLIKTNFSQVLWMDK
ARKEYMKESLRIRRLGNPEDCAGIVSFLCSEDASYITGETVVVGGGTASR
L

3D structure

• PDB: 2zat Molecular basis for peroxisomal localization of tetrameric carbonyl reductase.
• Chain: B
• Resolution: 1.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G25 S151 L161 Y164 K168 K209
• Catalytic site (residue number reindexed from 1): G16 S142 L152 Y155 K159 K200
• Enzyme Commision number: 1.1.1.184: carbonyl reductase (NADPH).
  1.1.1.300: NADP-retinol dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	B	A21 T23 D24 G25 I26 S45 R46 K47 N50 H71 N98 A100 V149 Y164 K168 P194 G195 I197 T199 F201 S202	A12 T14 D15 G16 I17 S36 R37 K38 N41 H62 N89 A91 V140 Y155 K159 P185 G186 I188 T190 F192 S193

Gene Ontology

Molecular Function

• GO:0000253 3-keto sterol reductase activity
• GO:0004090 carbonyl reductase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0042802 identical protein binding
• GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity

Biological Process

• GO:0001523 retinoid metabolic process
• GO:0008202 steroid metabolic process
• GO:0042180 cellular ketone metabolic process
• GO:0042572 retinol metabolic process
• GO:0042574 retinal metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005777 peroxisome

External links

• PDB: RCSB:2zat, PDBe:2zat, PDBj:2zat
• PDBsum: 2zat
• PubMed: 18334214
• UniProt: Q8WNV7|DHRS4_PIG Dehydrogenase/reductase SDR family member 4 (Gene Name=DHRS4)