Structure of PDB 2z8g Chain B

Receptor sequence

>2z8gB (length=549) Species: 5061 (Aspergillus niger)

REFMAVTANNSQLLTWWHNTGEINTQTPVADGNVRQSGLYSVKVQTTPAS
SSLYYDSFVYLAIPGNGMSDQLQYTQGYNQTQAWTSFLYSHDATVKISRN
GSSANSNVVIRPTSLNFPVRYDNQSVYITVPYSPTGYRFSVEFDDDLISL
APSGARQPENALLIFASPFENSSTKPQPGSPNSIAPAPGRVLGLNTTSAS
TVVFNPGVYYFTGHDHMVLSSSVTWVYFAPGAYVKGAVEFLSTASEVKAS
GHGVLSGEQYVWYADPDEGYQKASGANNNGLRMWRGTLGNSSQTFVLNGV
TVSAPPFNSMDWSGNSLDLITCRVDDYKQVGAFYGQTDGLEMYPGTILQD
VFYHTDDDGLKMYYSNVTARNIVMWKESVAPVVEFGWTPRNTENVLFDNV
DVIHQAYANAGNNPGIFGAVNNYLYAPDGLSSNHSTGNSNMTVRNITWSN
FRAEGSSSALFRINPIQNLDNISIKNVSIESFEPLSINTTESWMPVWYDL
NNGKQITVTDFSIEGFTVGNTTITASNAASVGRIDGVDPAYAGSVHYID

3D structure

• PDB: 2z8g Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D353 D372
• Catalytic site (residue number reindexed from 1): D338 D357
• Enzyme Commision number: 3.2.1.57: isopullulanase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	Y89 T90 G350	Y74 T75 G335
BS02	GLC	B	V394 G426 N427	V379 G411 N412
BS03	GLC	B	D372 W402 N428 D443	D357 W387 N413 D428

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0051675 isopullulanase activity

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:2z8g, PDBe:2z8g, PDBj:2z8g
• PDBsum: 2z8g
• PubMed: 18155243
• UniProt: O00105|IPUA_ASPNG Isopullulanase (Gene Name=ipuA)