Structure of PDB 2xxj Chain B

Receptor sequence

>2xxjB (length=309) Species: 300852 (Thermus thermophilus HB8)

MKVGIVGSGMVGSATAYALALLGVAREVVLVDLDRKLAQAHAEDILHATP
FAHPVWVWAGSYGDLEGARAVVLAAGVAQRPGETRLQLLDRNAQVFAQVV
PRVLEAAPEAVLLVATNPVDVMTQVAYALSGLPPGRVVGSGTILDTARFR
ALLAEYLRVAPQSVHAYVLGEHGDSEVLVWSSAQVGGVPLLEFAEARGRA
LSPEDRARIDEGVRRAAYRIIEGKGATYYGIGAGLARLVRAILTDEKGVY
TVSAFTPEVAGVLEVSLSLPRILGAGGVAGTVYPSLSPEERAALRRSAEI
LKEAAFALG

3D structure

• PDB: 2xxj Lactate Dehydrogenase from T. Thermophilus, Penta- Mutant (Ternary Complex)
• Chain: B
• Resolution: 1.964 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R85 D145 R148 H172
• Catalytic site (residue number reindexed from 1): R85 D145 R148 H172
• Enzyme Commision number: 1.1.1.27: L-lactate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	G9 M10 V11 D32 L33 Y62 A75 G76 V77 A78 V99 A115 N117 H172 T227 I231	G9 M10 V11 D32 L33 Y62 A75 G76 V77 A78 V99 A115 N117 H172 T227 I231

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006096 glycolytic process
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2xxj, PDBe:2xxj, PDBj:2xxj
• PDBsum: 2xxj
• UniProt: Q5SJA1|LDH_THET8 L-lactate dehydrogenase (Gene Name=ldh)