Structure of PDB 2xwn Chain B

Receptor sequence

>2xwnB (length=227) Species: 83332 (Mycobacterium tuberculosis H37Rv)

AGEVVAIVPAAGSGERLAVGVPKAFYQLDGQTLIERAVDGLLDSGVVDTV
VVAVPADRTDEARQILGHRAMIVAGGSNRTDTVNLALTVLSGTAEPEFVL
VHDAARALTPPALVARVVEALRDGYAAVVPVLPLSDTIKAVDANGVVLGT
PERAGLRAVQTPQGFTTDLLLRSYQRGSLDLPAAEYTDDASLVEHIGGQV
QVVDGDPLAFKITTKLDLLLAQAIVHH

3D structure

• PDB: 2xwn Structural and Functional Studies on Mycobacterial Ispd Enzymes
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.60: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CTP	B	P13 A14 A15 G16 G18 E19 R20 K27 N82 R83 T86 D107 A108 K215	P9 A10 A11 G12 G14 E15 R16 K23 N78 R79 T82 D103 A104 K211

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0002135 CTP binding
• GO:0003824 catalytic activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0030145 manganese ion binding
• GO:0050518 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
• GO:0070567 cytidylyltransferase activity

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
• GO:0051484 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway involved in terpenoid biosynthetic process

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:2xwn, PDBe:2xwn, PDBj:2xwn
• PDBsum: 2xwn
• PubMed: 21543842
• UniProt: P9WKG9|ISPD_MYCTU 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Gene Name=ispD)