Structure of PDB 2xcr Chain B

Receptor sequence

>2xcrB (length=718) Species: 158879 (Staphylococcus aureus subsp. aureus N315)

KLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEK
ARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTDADVDGAHI
RTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQ
TFEMLMGDVVENRRQFIEDNAVYANLDFAELPQSRINERNITSEMRESFL
DYAMSVIVARALPDVRDGLKPVHRRILYGLNEQGMTPDKSYKKSARIVGD
VMGKYHPHGDSSIYEAMVRMAQDFSYRYPLVDGQGNFGSMDGDGAAAMRF
TEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVLPARFPNLLANGAS
GIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDFPTAGL
ILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARM
IEKIAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYK
QTPLQTSFGVNMIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRK
AKDRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQ
AILDMRLRRLTGLERDKIEAEYNELLNYISELETILADEEVLLQLVRDEL
TEIRDRFGDDRRTEIQLG

3D structure

• PDB: 2xcr Type Iia Topoisomerase Inhibition by a New Class of Antibacterial Agents.
• Chain: B
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	E435 D437 S438 G459 K460 K581 R1033 V1045 H1046 H1079 H1081 R1092 R1272	E19 D21 S22 G43 K44 K165 R260 V272 H273 H306 H308 R319 R499
BS02	dna	B	K460 L462 N463 K466 R471 H515 V626 R629 R1122 F1123 I1175 V1177 G1178 M1179 R1238	K44 L46 N47 K50 R55 H99 V210 R213 R349 F350 I402 V404 G405 M406 R465

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:2xcr, PDBe:2xcr, PDBj:2xcr
• PDBsum: 2xcr
• PubMed: 20686482
• UniProt: P66937|GYRB_STAAN DNA gyrase subunit B (Gene Name=gyrB);
  Q99XG5|GYRA_STAAN DNA gyrase subunit A (Gene Name=gyrA)