Structure of PDB 2x0r Chain B

Receptor sequence

>2x0rB (length=303) Species: 2238 (Haloarcula marismortui)

TKVSVVGAAGTVGAAAGYNIALRDIADEVVFVDIPDKEDDTVGQAADTNH
GIAYDSNTRVRQGGYEDTAGSDVVVITAGIPRQPGQTRIDLAGDNAPIME
DIQSSLDEHNDDYISLTTSNPVDLLNRHLYEAGDRSREQVIGFGGRLDSA
RFRYVLSEEFDAPVQNVEGTILGEHGDAQVPVFSKVSVDGTDPEFSGDEK
EQLLGDLQESAMDVIERKGATEWGPARGVAHMVEAILHDTGEVLPASVKL
EGEFGHEDTAFGVPVSLGSNGVEEIVEWDLDDYEQDLMADAAEKLSDQYD
KIS

3D structure

• PDB: 2x0r The Oligomeric States of Haloarcula Marismortui Malate Dehydrogenase are Modulated by Solvent Components as Shown by Crystallographic and Biochemical Studies
• Chain: B
• Resolution: 2.915 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R109 D168 R171 H195
• Catalytic site (residue number reindexed from 1): R88 D148 R151 H175
• Enzyme Commision number: 1.1.1.37: malate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	G28 G30 T31 V32 D53 T97 A98 G99 R102 T138 S139 N140 F163 H195	G7 G10 T11 V12 D33 T77 A78 G79 R82 T118 S119 N120 F143 H175
BS02	CL	B	K205 D306	K185 D279
BS03	CL	B	R43 R252 A255 H256 E259	R23 R227 A230 H231 E234

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0030060 L-malate dehydrogenase (NAD+) activity

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006099 tricarboxylic acid cycle
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2x0r, PDBe:2x0r, PDBj:2x0r
• PDBsum: 2x0r
• PubMed: 12581646
• UniProt: Q07841|MDH_HALMA Malate dehydrogenase (Gene Name=mdh)