Structure of PDB 2wtf Chain B

Receptor sequence

>2wtfB (length=508) Species: 4932 (Saccharomyces cerevisiae)

MSKFTWKELIQLGSPSKAYESSLACIAHIDMNAFFAQVEQMRCGLSKEDP
VVCVQWNSIIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFKKGED
FWQYHDGCGSWVQDPAKQISVEDHKVSLEPYRRESRKALKIFKSACDLVE
RASIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIGGNYD
INSHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCKGIRD
SIKDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLDCGKF
EITSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFLDAKV
KQSDYDRSTSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMMSNKN
LRGKSCNSIVDCISWLEVFCAELTSRIQDLEQEYNKIVIPRTVSISLKTK
SYEVYRKSGPVAYKGINFQSHELLKVGIKFVTDLDIKGKNKSYYPLTKLS
MTITNFDI

3D structure

• PDB: 2wtf Crystal Structure of a Cisplatin-(1,3-Gtg) Cross-Link within DNA Polymerase Eta.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R73 M74 E79 K125 K393 S394 M396 N398 R426	R73 M74 E79 K125 K393 S394 M396 N398 R426
BS02	dna	B	S153 I154 V311 R456	S153 I154 V311 R456
BS03	CA	B	D30 D155 E156	D30 D155 E156
BS04	CA	B	D30 M31 D155	D30 M31 D155
BS05	DTP	B	N32 A33 F34 F35 I60 Y64 R67 R73 D155	N32 A33 F34 F35 I60 Y64 R67 R73 D155
BS06	CA	B	G98 E417	G98 E417
BS07	CA	B	D318 D321	D318 D321
BS08	CA	B	G298 E325	G298 E325

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2wtf, PDBe:2wtf, PDBj:2wtf
• PDBsum: 2wtf
• PubMed: 20333640
• UniProt: Q04049|POLH_YEAST DNA polymerase eta (Gene Name=RAD30)