Structure of PDB 2rab Chain B

Receptor sequence

>2rabB (length=452) Species: 1048 (Marichromatium gracile) [Search protein sequence]

TQHFDLIAIGGGSGGLAVAEKAAAFGKRVALIESKALGGTCVNVGCVPKK
VMWYASHLAEAVRDAPGFGVQASTLDWPRLVAGRDRYIGAINSFWDGYVE
RLGITRVDGHARFVDAHTIEVEGQRLSADHIVIATGGRPIVPRLPGAELG
ITSDGFFALQQQPKRVAIIGAGYIGIELAGLLRSFGSEVTVVALEDRLLF
QFDPLLSATLAENMHAQGIETHLEFAVAALERDAQGTTLVAQDGTRLGFD
SVIWAVGRAPNTRDLGLEAAGIEVQSNGMVPTDAYQNTNVPGVYALGDIT
GRDQLTPVAIAAGRRLAERLFDGQSERKLDYDNIPTVVFAHPPLSKVGLS
EPEARERLGDVLTVYETSFTPMRYALNEHGPKTAMKLVCAGPEQRVVGVH
VIGDGADEMLQGFAVAVKMGATKADFDNTVAIHPGSAEELVTLKEPVRRP
GD

3D structure

PDB

2rab Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase.

Chain

Resolution

2.5 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	S14 L38 C42 C47 K50 Y176 E180 I314 A435 H437 E442
Catalytic site (residue number reindexed from 1)	S13 L37 C41 C46 K49 Y173 E177 I310 A431 H433 E438
Enzyme Commision number	1.8.1.16: glutathione amide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	G11 G13 S14 G15 E34 S35 T41 G46 C47 K50 H113 A114 T138 G139 R262 G301 D302 Q308 L309 T310	G10 G12 S13 G14 E33 S34 T40 G45 C46 K49 H110 A111 T135 G136 R258 G297 D298 Q304 L305 T306
BS02	NAD	B	K50 I172 G175 Y176 I177 E180 A196 L197 E198 V260 G261 Q308 V341	K49 I169 G172 Y173 I174 E177 A193 L194 E195 V256 G257 Q304 V337

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004362	glutathione-disulfide reductase (NADPH) activity
GO:0016491	oxidoreductase activity
GO:0016668	oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0046872	metal ion binding
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

	Biological Process
GO:0006749	glutathione metabolic process
GO:0034599	cellular response to oxidative stress
GO:0045454	cell redox homeostasis
GO:0098869	cellular oxidant detoxification

	Cellular Component
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2rab, PDBe:2rab, PDBj:2rab
PDBsum	2rab
PubMed	17977556
UniProt	D0VWY5\|GASHR_MARGR Glutathione amide reductase (Gene Name=garB)

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