Structure of PDB 2r0g Chain B

Receptor sequence

>2r0gB (length=518) Species: 68170 (Lentzea aerocolonigenes)

APIETDVLILGGGPVGMALALDLAHRQVGHLVVEQTDGTITHPRVGTIGP
RSMELFRRWGVAKQIRTAGWPGDHPLDAAWVTRVGGHEVYRIPLGTADTR
ATPEHTPEPDAICPQHWLAPLLAEAVGERLRTRSRLDSFEQRDDHVRATI
TDLRTGATRAVHARYLVACDGASSPTRKALGIDAPPRHRTQVFRNILFRA
PELRSLLGERAALFFFLMLSSSLRFPLRALDGRGLYRLTVGVDTMDSFEL
VRRAVAFDTEIEVLSDSEWHLTHRVADSFSAGRVFLTGDAAHTLSPSGGF
GMNTGIGSAADLGWKLAATLRGWAGPGLLATYEEERRPVAITSLEEANVN
LRRTMDRELPPGLHDDGPRGERIRAAVAEKLERSGARREFDAPGIHFGHT
YRSSIVCGEPATGGWRPSARPGARAPHAWLTPTTSTLDLFGRGFVLLSFG
TTDGVEAVTRAFADRHVPLETVTCHAPEIHALYERAHVLVRPDGHVAWRG
DHLPAELGGLVDKVRGAA

3D structure

• PDB: 2r0g Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC
• Chain: B
• Resolution: 2.37 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G48 L229 T241 P303
• Catalytic site (residue number reindexed from 1): G46 L227 T239 P296

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	L12 P16 V17 E36 Q37 R46 V47 G48 T49 L138 D172 G173 W276 G295 D296 G308 M309 N310	L10 P14 V15 E34 Q35 R44 V45 G46 T47 L136 D170 G171 W269 G288 D289 G301 M302 N303
BS02	7CK	B	F216 F227 P228 R230 R239 P303 S304 G305 E396 F397	F214 F225 P226 R228 R237 P296 S297 G298 E389 F390

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004497 monooxygenase activity
• GO:0008168 methyltransferase activity
• GO:0016491 oxidoreductase activity
• GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
• GO:0071949 FAD binding

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:2r0g, PDBe:2r0g, PDBj:2r0g
• PDBsum: 2r0g
• PubMed: 17873060
• UniProt: Q8KI25