Structure of PDB 2qpu Chain B

Receptor sequence

>2qpuB (length=404) Species: 4513 (Hordeum vulgare)

HQVLFQGFNWESWKQSGGWYNMMMGKVDDIAAAGVTHVWLPPPSHSVSNE
GYMPGRLYDIDASKYGNAAELKSLIGALHGKGVQAIADIVINHRCADYKD
SRGIYCIFEGGTSDGRLDWGPHMICRDDTKYSDGTANLDTGADFAAAPDI
DHLNDRVQRELKEWLLWLKSDLGFDAWRLDFARGYSPEMAKVYIDGTSPS
LAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDKVGGAASAGMVFDFTT
KGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAVTFVDNHDTGSTQAMWP
FPSDKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITA
TSALKILMHEGDAYVAEIDGKVVVKIGPRYDVGAVIPAGFVTSAHGNDYA
VWEK

3D structure

• PDB: 2qpu The 'pair of sugar tongs' site on the non-catalytic domain C of barley alpha-amylase participates in substrate binding and activity
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D180 E205 D291
• Catalytic site (residue number reindexed from 1): D180 E205 D291
• Enzyme Commision number: 3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	B	H395 D398	H395 D398
BS02	AC1	B	K375 Y380 D381 V382 Y399 A400 W402	K375 Y380 D381 V382 Y399 A400 W402
BS03	CA	B	N92 D139 A142 D149 G184	N92 D139 A142 D149 G184
BS04	CA	B	E109 T112 D114 D118	E109 T112 D114 D118
BS05	CA	B	D128 D143 F144 A147 P148 D149	D128 D143 F144 A147 P148 D149
BS06	QPU	B	Q227 V230 D234 W278 W279	Q227 V230 D234 W278 W279

Gene Ontology

Molecular Function

• GO:0004556 alpha-amylase activity
• GO:0005509 calcium ion binding
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005983 starch catabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:2qpu, PDBe:2qpu, PDBj:2qpu
• PDBsum: 2qpu
• PubMed: 17803687
• UniProt: P00693|AMY1_HORVU Alpha-amylase type A isozyme (Gene Name=AMY1.1)