Structure of PDB 2px8 Chain B

Receptor sequence

>2px8B (length=265) Species: 301478 (Murray valley encephalitis virus (strain MVE-1-51))

GRTLGEQWKEKLNAMGKEEFFSYRKEAILEVDRTEARRARREGNKVGGHP
VSRGTAKLRWLVERRFVQPIGKVVDLGCGRGGWSYYAATMKNVQEVRGYT
KGGPGHEEPMLMQSYGWNIVTMKSGVDVFYKPSEISDTLLCDIGESSPSA
EIEEQRTLRILEMVSDWLSRGPKEFCIKILCPYMPKVIEKLESLQRRFGG
GLVRVPLSRNSNHEMYWVSGASGNIVHAVNMTSQVLIGRMDKKIWKGPKY
EEDVNLGSGTRAVGK

3D structure

• PDB: 2px8 Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.91: flavivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MGT	B	K13 L16 N17 M19 K21 F24 R28 S151 P152 R213 S215	K9 L12 N13 M15 K17 F20 R24 S147 P148 R209 S211
BS02	MGT	B	E22 F25	E18 F21
BS03	3PO	B	R37 R41 S56 R57 R84	R33 R37 S52 R53 R80
BS04	SAH	B	S56 G58 G81 G86 W87 X105 H110 E111 V130 D131 V132 F133 D146	S52 G54 G77 G82 W83 X101 H106 E107 V126 D127 V128 F129 D142

Gene Ontology

Molecular Function

• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
• GO:0008168 methyltransferase activity

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:2px8, PDBe:2px8, PDBj:2px8
• PDBsum: 2px8
• PubMed: 17622627
• UniProt: P05769|POLG_MVEV5 Genome polyprotein