Structure of PDB 2oxi Chain B

Receptor sequence

>2oxiB (length=374) Species: 9796 (Equus caballus)

STAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRSDD
HVVSGTLVTPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKC
RVCKHPEGNFCLKNDLSMPRGTMQDGTSRFTCRGKPIHHFLGTSTFSQYT
VVDEISVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTQGSTCAVFGL
GGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATECVNPQDYKKPI
QEVLTEMSNGGVDFSFEVIGRLDTMVTALSCCQEAYGVSVIVGVPPDSQN
LSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVL
PFEKINEGFDLLRSGESIRTILTF

3D structure

• PDB: 2oxi Refined structure of Cu-substituted alcohol dehydrogenase at 2.1 A resolution.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C46 R47 S48 H51 H67 E68 C97 C100 C103 C111 D115 C174 T178 R369
• Catalytic site (residue number reindexed from 1): C46 R47 S48 H51 H67 E68 C97 C100 C103 C111 D115 C174 T178 R369
• Enzyme Commision number: 1.1.1.1: alcohol dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU	B	C46 H67	C46 H67
BS02	ZN	B	C97 C100 C103 C111	C97 C100 C103 C111
BS03	NAD	B	C46 R47 S48 H51 C174 T178 G201 V203 D223 I224 K228 V268 I269 V292 V294 I318	C46 R47 S48 H51 C174 T178 G201 V203 D223 I224 K228 V268 I269 V292 V294 I318
BS04	DMS	B	S48 H67	S48 H67

Gene Ontology

Molecular Function

• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:2oxi, PDBe:2oxi, PDBj:2oxi
• PDBsum: 2oxi
• PubMed: 15299812
• UniProt: P00327|ADH1E_HORSE Alcohol dehydrogenase E chain