Structure of PDB 2onh Chain B

Receptor sequence

>2onhB (length=543) Species: 29719 (Mentha spicata)

MRRSGNYNPSRWDVNFIQSLLSDYKEDKHVIRASELVTLVKMELEKETDQ
IRQLELIDDLQRMGLSDHFQNEFKEILSSIYLDHHYYKNPFPKEERDLYS
TSLAFRLLREHGFQVAQEVFDSFKNEEGEFKESLSDDTRGLLQLYEASFL
LTEGETTLESAREFATKFLEEKVNEGGVDGDLLTRIAYSLDIPLHWRIKR
PNAPVWIEWYRKRPDMNPVVLELAILDLNIVQAQFQEELKESFRWWRNTG
FVEKLPFARDRLVECYFWNTGIIEPRQHASARIMMGKVNALITVIDDIYD
VYGTLEELEQFTDLIRRWDINSIDQLPDYMQLCFLALNNFVDDTSYDVMK
EKGVNVIPYLRQSWVDLADKYMVEARWFYGGHKPSLEEYLENSWQSISGP
CMLTHIFFRVTDSFTKETVDSLYKYHDLVRWSSFVLRLADDLGTSVEEVS
RGDVPKSLQCYMSDYNASEAEARKHVKWLIAEVWKKMNAERVSKDSPFGK
DFIGCAVDLGRMAQLMYHNGDGHGTQHPIIHQQMTRTLFEPFA

3D structure

• PDB: 2onh Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R315 W324 N345 D352 D356 S452 I453 S454 R493 D496 T500 E504 Y573 D577 H579
• Catalytic site (residue number reindexed from 1): R259 W268 N289 D296 D300 S396 I397 S398 R437 D440 T444 E448 Y517 D521 H523

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	B	D352 D356 Y427	D296 D300 Y371
BS02	MN	B	D352 D356	D296 D300
BS03	MN	B	D496 T500 K512	D440 T444 K456
BS04	F3P	B	R315 W324 N345 D352 M458 R493 D496 Y573 H579	R259 W268 N289 D296 M402 R437 D440 Y517 H523

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0010333 terpene synthase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006721 terpenoid metabolic process
• GO:0016102 diterpenoid biosynthetic process

Cellular Component

• GO:0009507 chloroplast

External links

• PDB: RCSB:2onh, PDBe:2onh, PDBj:2onh
• PDBsum: 2onh
• PubMed: 17372193
• UniProt: Q40322