Structure of PDB 2oh2 Chain B

Receptor sequence

>2oh2B (length=440) Species: 9606 (Homo sapiens)

LNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQ
KAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDN
PELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPN
FDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRR
YFIKNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKN
KPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELY
QQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINK
AEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSV
VSTAEEIFAIAKELLKTEIDADFPHPLRLRLMGVRISSFP

3D structure

• PDB: 2oh2 Ternary complex of the catalytic core of human DNA polymerase Kappa with DNA and dTT
• Chain: B
• Resolution: 3.05 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	S196 K321 G356 I357 G358 V360 T361 R454 T469 A471 S472 T473	S176 K244 G279 I280 G281 V283 T284 R377 T392 A394 S395 T396
BS02	dna	B	T44 F49 S134 M135 P153 I156 S388 T390 R413 K414 S415 M416 S417 V418 E419 R420 T421 R507	T24 F29 S114 M115 P133 I136 S311 T313 R336 K337 S338 M339 S340 V341 E342 R343 T344 R430
BS03	MG	B	D107 M108 D198	D87 M88 D178
BS04	TTP	B	D107 M108 D109 A110 F111 Y112 S137 T138 Y141 R144 D198 K328	D87 M88 D89 A90 F91 Y92 S117 T118 Y121 R124 D178 K251

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2oh2, PDBe:2oh2, PDBj:2oh2
• PDBsum: 2oh2
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)