Structure of PDB 2o54 Chain B

Receptor sequence

>2o54B (length=631) Species: 562 (Escherichia coli) [Search protein sequence]

MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGHLLEQAQ
PDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVH
AGDPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLR
SNSEFVPLCVSALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQT
PVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIWQPSEACEPYQ
DEEGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIE
AAKRFGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNA
ISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEA
KVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAM
TGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHS
TDAGKALFHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY
QLIDQAKRTPVRQFRGIVAPGSGAIAHHHHH

3D structure

PDB

2o54 Structural Studies of E. coli Topoisomerase III-DNA Complexes Reveal a Novel Type IA Topoisomerase-DNA Conformational Intermediate.

Chain

Resolution

2.5 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	E7 D105 E107
Catalytic site (residue number reindexed from 1)	E7 D105 E107
Enzyme Commision number	5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	E7 K8 G43 H44 Q50 P51 W61 E107 R165 D169 W170 G173 I174 T177 R178 R185 S194 V195 G196 R197 V198 Q199 E321 Y328 R330 T524 T527 R538	E7 K8 G43 H44 Q50 P51 W61 E107 R165 D169 W170 G173 I174 T177 R178 R185 S194 V195 G196 R197 V198 Q199 E321 Y328 R330 T524 T527 R538

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity
GO:0005515	protein binding
GO:0046872	metal ion binding
GO:0098847	sequence-specific single stranded DNA binding

	Biological Process
GO:0006261	DNA-templated DNA replication
GO:0006265	DNA topological change
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0051304	chromosome separation

	Cellular Component
GO:0043597	cytoplasmic replication fork

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2o54, PDBe:2o54, PDBj:2o54
PDBsum	2o54
PubMed	17331537
UniProt	P14294\|TOP3_ECOLI DNA topoisomerase 3 (Gene Name=topB)

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