Structure of PDB 2nvu Chain B

Receptor sequence
>2nvuB (length=789) Species: 9606 (Homo sapiens) [Search protein sequence]
KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQV
AATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYN
GKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFN
LQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIK
NKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKVAL
KSYEEEATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALA
AAQTNAAADWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLV
IGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPK
AEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRW
INGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECT
LELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDD
PEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVC
ATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNI
QFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEER
TRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS
3D structure
PDB2nvu Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.
ChainB
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K2021 D2146 C2216 T2217 A2219
Catalytic site (residue number reindexed from 1) K368 D493 C563 T564 A566
Enzyme Commision number 6.2.1.64: E1 NEDD8-activating enzyme.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN B C2199 C2343 C2346 C546 C690 C693
BS02 ATP B G2057 D2079 M2080 D2081 K2103 K2126 I2127 Q2128 L2145 D2146 G404 D426 M427 D428 K450 K473 I474 Q475 L492 D493
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008641 ubiquitin-like modifier activating enzyme activity
GO:0015144 carbohydrate transmembrane transporter activity
GO:0016874 ligase activity
GO:0019781 NEDD8 activating enzyme activity
GO:0019788 NEDD8 transferase activity
GO:0042802 identical protein binding
GO:0046982 protein heterodimerization activity
Biological Process
GO:0000278 mitotic cell cycle
GO:0006508 proteolysis
GO:0007113 endomitotic cell cycle
GO:0008643 carbohydrate transport
GO:0036211 protein modification process
GO:0043687 post-translational protein modification
GO:0045116 protein neddylation
GO:0051726 regulation of cell cycle
GO:0055085 transmembrane transport
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2nvu, PDBe:2nvu, PDBj:2nvu
PDBsum2nvu
PubMed17220875
UniProtQ8TBC4|UBA3_HUMAN NEDD8-activating enzyme E1 catalytic subunit (Gene Name=UBA3)

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