Structure of PDB 2jlx Chain B

Receptor sequence

>2jlxB (length=451) Species: 408688 (Dengue virus 4 Thailand/0348/1991)

GSAMGEPDYEVDEDIFRKKRLTIMDLHPGAGKTKRILPSIVREALKRRLR
TLILAPTRVVAAEMEEALRGLPIRYQTPAVKSDHTGREIVDLMCHATFTT
RLLSSTRVPNYNLIVMDEAHFTDPCSVAARGYISTRVEMGEAAAIFMTAT
PPGSIDPFPQSNSPIEDIEREIPERSWNTGFDWITDYQGKTVWFVPSIKA
GNDIANCLRKSGKRVIQLSRKTFDTEYPKTKLTDWDFVVTTDISEMGANF
RAGRVIDPRRCLKPVILTDGPERVILAGPIPVTPASAAQRRGRIGRNPAQ
EDDQYVFSGDPLKNDEDHAHWTEAKMLLDNIYTPEGIIPTLFGPEREKTQ
AIDGEFRLRGEQRKTFVELMRRGDLPVWLSYKVASAGISYKDREWCFTGE
RNNQILEENMEVEIWTREGEKKKLRPKWLDARVYADPMALKDFKEFASGR
K

3D structure

• PDB: 2jlx Insights Into RNA Unwinding and ATP Hydrolysis by the Flavivirus Ns3 Protein.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.91: flavivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	T224 R225 R241 Q243 T244 P245 C261 A263 T264 R268 T273 D290 P363 S364 I365 S386 R387 T408 I410 L443 R599	T57 R58 R74 Q76 T77 P78 C94 A96 T97 R101 T106 D123 P196 S197 I198 S219 R220 T241 I243 L276 R432
BS02	ADP	B	G196 G198 K199 T200 K201 N416 R418 R463	G29 G31 K32 T33 K34 N249 R251 R296

Gene Ontology

Molecular Function

• GO:0004386 helicase activity
• GO:0005524 ATP binding

External links

• PDB: RCSB:2jlx, PDBe:2jlx, PDBj:2jlx
• PDBsum: 2jlx
• PubMed: 19008861
• UniProt: Q2YHF0|POLG_DEN4T Genome polyprotein