Structure of PDB 2iwh Chain B

Receptor sequence

>2iwhB (length=980) Species: 4932 (Saccharomyces cerevisiae)

HHHHSDSQQSIKVLEELFQKLSVATADNRHEIASEVASFLNGNIIEHDVP
EHFFGELAKGIKDKKTAANAMQAVAHIANQSNLSPSVEPYIVQLVPAICT
NAGNKDKEIQSVASETLISIVNAVNPVAIKALLPHLTNAIVETNKWQEKI
AILAAFSAMVDAAKDQVALRMPELIPVLSETMWDTKKEVKAAATAAMTKA
TETVDNKDIERFIPSLIQCIADPTEVPETVHLLGATTFVAEVTPATLSIM
VPLLSRGLNERETGIKRKSAVIIDNMCKLVEDPQVIAPFLGKLLPGLKSN
FATIADPEAREVTLRALKTLRRVGNVGEDDAIPELSHAGDVSTTLQVVNE
LLKDETVAPRFKIVVEYIAAIGADLIDERIIDQQAWFTHITPYMTIFLHE
KKAKDILDEFRKRAVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAKILLN
KTQLRLKRARRYGICGPNGCGKSTLMRAIANGQVDGFPTQEECRTVYVEH
DIDGTHSDTSVLDFVFESGVGTKEAIKDKLIEFGFTDEMIAMPISALSGG
WKMKLALARAVLRNADILLLDEPTNHLDTVNVAWLVNYLNTCGITSITIS
HDSVFLDNVCEYIINYEGLKLRKYKGNFTEFVKKCPAAKAYEELSNTDLE
FKFPEPGYLEGVKTKQKAIVKVTNMEFQYPGTSKPQITDINFQCSLSSRI
AVIGPNGAGKSTLINVLTGELLPTSGEVYTHENCRIAYIKQHAFAHIESH
LDKTPSEYIQWRFQTGEDRETMDRANRQINENDAEAMNKIFKIEGTPRRI
AGIHSRRKFKNTYEYECSFLLGENIGMKSERWVPMMSVDNAWIPRGELVE
SHSKMVAEVDMKEALASGQFRPLTRKEIEEHCSMLGLDPEIVSHSRIRGL
SGGQKVKLVLAAGTWQRPHLIVLDEPTNYLDRDSLGALSKALKEFEGGVI
IITHSAEFTKNLTEEVWAVKDGRMTPSGHN

3D structure

• PDB: 2iwh Structure of Eef3 and the Mechanism of Transfer RNA Release from the E-Site.
• Chain: B
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	B	I41 H43 D44 S82 T391 I392 H395 E396 N702 E921 N924 H950	I45 H47 D48 S86 T395 I396 H399 E400 N706 E925 N928 H954

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0019843 rRNA binding
• GO:0043022 ribosome binding

Biological Process

• GO:0001933 negative regulation of protein phosphorylation
• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0006415 translational termination
• GO:0006469 negative regulation of protein kinase activity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0010494 cytoplasmic stress granule
• GO:0022626 cytosolic ribosome

External links

• PDB: RCSB:2iwh, PDBe:2iwh, PDBj:2iwh
• PDBsum: 2iwh
• PubMed: 16929303
• UniProt: P16521|EF3A_YEAST Elongation factor 3A (Gene Name=YEF3)