Structure of PDB 2iwf Chain B

Receptor sequence

>2iwfB (length=590) Species: 223 (Achromobacter cycloclastes)

ADGSVAPGKLDDYYGFWSSGQTGEMRILGIPSMRELMRVPVFNRCSATGW
GQTNESIRIHQRTMTEKTKKQLAANGKKIHDNGDLHHVHMSFTDGKYDGR
YLFMNDKANTRVARVRCDVMKTDAILEIPNAKGIHGMRPQKWPRSNYVFC
NGEDEAPLVNDGSTMTDVATYVNIFTAVDADKWEVAWQVKVSGNLDNCDA
DYEGKWAFSTSYNSEMGMTLEEMTKSEMDHVVVFNIAEIEKAIKAGQYEE
INGVKVVDGRKEAKSLFTRYIPIANNPHGCNMAPDRKHLCVAGKLSPTVT
VLDVTKFDALFYDNAEPRSAVVAEPELGLGPLHTAFDGRGNAYTSLFLDS
QVVKWNIDEAIRAYAGEKINPIKDKLDVQYQPGHLKTVMGETLDAANDWL
VCLCKFSKDRFLNVGPLKPENDQLIDISGDKMVLVHDGPTFAEPHDAIAV
SPSILPNIRSVWDRNDPLWAETRKQAEADEVDIDEWTEAVIRDGNKVRVY
MTSVAPSFSQPSFTVKEGDEVTVIVTNLDEIDDLTHGFTMGNHGVAMEVG
PQQTSSVTFVAANPGVYWYYCQWFCHALHMEMRGRMFVEP

3D structure

• PDB: 2iwf Insight Into Catalysis of Nitrous Oxide Reductase from High-Resolution Structures of Resting and Inhibitor-Bound Enzyme from Achromobacter Cycloclastes.
• Chain: B
• Resolution: 1.86 Å

Enzymatic activity

• Enzyme Commision number: 1.7.2.4: nitrous-oxide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	R51 S53	R44 S46
BS02	CU	B	H543 C578 C582 M589	H536 C571 C575 M582
BS03	CU	B	C578 W580 C582 H586	C571 W573 C575 H579
BS04	CA	B	K412 E427	K405 E420
BS05	CA	B	Y219 E222 M230 T231 D236 N283	Y212 E215 M223 T224 D229 N276
BS06	NA	B	R123 K128 T129 D130 L475	R116 K121 T122 D123 L468
BS07	NA	B	K248 K251 A252	K241 K244 A245
BS08	NA	B	I66 R69 D130 A131	I59 R62 D123 A124
BS09	NA	B	N50 R51 I86 H87 D88	N43 R44 I79 H80 D81
BS10	NA	B	R51 C52 S53 G56 Q59 A553	R44 C45 S46 G49 Q52 A546
BS11	NA	B	F49 H67 H87 D88 N89 R118	F42 H60 H80 D81 N82 R111

Gene Ontology

Molecular Function

• GO:0004129 cytochrome-c oxidase activity
• GO:0005507 copper ion binding
• GO:0005509 calcium ion binding
• GO:0050304 nitrous-oxide reductase activity

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:2iwf, PDBe:2iwf, PDBj:2iwf
• PDBsum: 2iwf
• PubMed: 16904686
• UniProt: P94127|NOSZ_ACHCY Nitrous-oxide reductase (Gene Name=nosZ)