Structure of PDB 2iw4 Chain B

Receptor sequence
>2iw4B (length=308) Species: 1423 (Bacillus subtilis) [Search protein sequence]
MEKILIFGHQNPDTDTICSAIAYADLKNKLGFNAEPVRLGQVNGETQYAL
DYFKQESPRLVETAANEVNGVILVDHNERQQSIKDIEEVQVLEVIDHQRI
ANFETAEPLYYRAEPVGCTATILNKMYKENNVKIEKEIAGLMLSAIISDS
LLFKSPTCTDQDVAAAKELAEIAGVDAEEYGLNMLKAGADLSKKTVEELI
SLDAKEFTLGSKKVEIAQVNTVDIEDVKKRQAELEAVISKVVAEKNLDLF
LLVITDILENDSLALAIGNEAAKVEKAFNVTLENNTALLKGVVSRKKQVV
PVLTDAMA
3D structure
PDB2iw4 A Trimetal Site and Substrate Distortion in a Family II Inorganic Pyrophosphatase.
ChainB
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.1.1: inorganic diphosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE B H9 D13 D75 H9 D13 D75
BS02 FE B D15 D75 H97 D149 D15 D75 H97 D149
BS03 MG B D13 D149 D13 D149
BS04 MN B D15 D75 H97 D149 D15 D75 H97 D149
BS05 2PN B D13 H97 Q98 D149 K205 R295 K296 D13 H97 Q98 D149 K205 R295 K296 MOAD: Kd=12uM
Gene Ontology
Molecular Function
GO:0004427 inorganic diphosphate phosphatase activity
GO:0016462 pyrophosphatase activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Cellular Component
External links
PDB RCSB:2iw4, PDBe:2iw4, PDBj:2iw4
PDBsum2iw4
PubMed17095506
UniProtP37487|PPAC_BACSU Manganese-dependent inorganic pyrophosphatase (Gene Name=ppaC)

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