Structure of PDB 2is6 Chain B

Receptor sequence

>2is6B (length=652) Species: 562 (Escherichia coli) [Search protein sequence]

DVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVEN
CSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAH
HMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKD
EGLRPHHINPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPH
ILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYG
WRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLG
KKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYR
SNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIVNRNDDA
AFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAA
SALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTR
IENLEELVTATRQFSEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKL
TLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMG
TP

3D structure

PDB

2is6 UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.

Chain

Resolution

2.2 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	T36 D220 Q251
Catalytic site (residue number reindexed from 1)	T35 D215 Q246
Enzyme Commision number	5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	Y621 G622	Y611 G612
BS02	dna	B	T63 N64 F189 Y254 W256 R355 S356 N357 Y377 N412 T415 G417 G419 R421 T422 L540 T558 H560 S561 S582 M584 Y621	T62 N63 F184 Y249 W251 R350 S351 N352 Y372 N407 T410 G412 G414 R416 T417 L530 T548 H550 S551 S572 M574 Y611
BS03	MGF	B	A31 K35 E221 Q251 R284 R605	A30 K34 E216 Q246 R279 R595
BS04	ADP	B	S9 Q14 G32 G34 K35 T36 R37 R73 Y283 R284 E566	S8 Q13 G31 G33 K34 T35 R36 R72 Y278 R279 E556

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0015616	DNA translocase activity
GO:0016787	hydrolase activity
GO:0016853	isomerase activity
GO:0016887	ATP hydrolysis activity
GO:0017116	single-stranded DNA helicase activity
GO:0042803	protein homodimerization activity
GO:0043138	3'-5' DNA helicase activity

	Biological Process
GO:0000717	nucleotide-excision repair, DNA duplex unwinding
GO:0000725	recombinational repair
GO:0006260	DNA replication
GO:0006268	DNA unwinding involved in DNA replication
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006298	mismatch repair
GO:0009314	response to radiation
GO:0009432	SOS response
GO:0031297	replication fork processing
GO:0032508	DNA duplex unwinding
GO:0070581	rolling circle DNA replication
GO:0070716	mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0017117	single-stranded DNA-dependent ATP-dependent DNA helicase complex
GO:0033202	DNA helicase complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:2is6, PDBe:2is6, PDBj:2is6
PDBsum	2is6
PubMed	17190599
UniProt	P03018\|UVRD_ECOLI DNA helicase II (Gene Name=uvrD)

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