Structure of PDB 2is4 Chain B

Receptor sequence

>2is4B (length=632) Species: 562 (Escherichia coli) [Search protein sequence]

MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVE
NCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRA
HHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQK
DEGLRPHHIGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNK
PHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSI
YGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGR
LGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAIL
YRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIVNRND
DAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGR
AASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGQTRI
ENLEELVTATRQFSLMPLQAFLSHADTWQDAVQLMTLHSAKGLEFPQVFI
VGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYG
KEVYHRPSRFIGELPEECVEEVRLRVSRPVQR

3D structure

PDB

2is4 UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.

Chain

Resolution

2.6 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	T36 D220 Q251
Catalytic site (residue number reindexed from 1)	T36 D217 Q248
Enzyme Commision number	5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	F62 T63 N64 H91 I113 F189 W256 R355 S356 N357 M380 G417 G419 R421 T422 S536 T558 H560 M584 Y621	F62 T63 N64 H91 I113 F186 W253 R352 S353 N354 M377 G414 G416 R418 T419 S523 T536 H538 M562 Y599
BS02	dna	B	R453 Y621 G622	R450 Y599 G600
BS03	ANP	B	S9 Q14 A31 G32 G34 K35 T36 R37 R73 Q251 Y283 R284 E566 R605	S9 Q14 A31 G32 G34 K35 T36 R37 R73 Q248 Y280 R281 E544 R583

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0015616	DNA translocase activity
GO:0016787	hydrolase activity
GO:0016853	isomerase activity
GO:0016887	ATP hydrolysis activity
GO:0017116	single-stranded DNA helicase activity
GO:0042803	protein homodimerization activity
GO:0043138	3'-5' DNA helicase activity

	Biological Process
GO:0000717	nucleotide-excision repair, DNA duplex unwinding
GO:0000725	recombinational repair
GO:0006260	DNA replication
GO:0006268	DNA unwinding involved in DNA replication
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006298	mismatch repair
GO:0009314	response to radiation
GO:0009432	SOS response
GO:0031297	replication fork processing
GO:0032508	DNA duplex unwinding
GO:0070581	rolling circle DNA replication
GO:0070716	mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0017117	single-stranded DNA-dependent ATP-dependent DNA helicase complex
GO:0033202	DNA helicase complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:2is4, PDBe:2is4, PDBj:2is4
PDBsum	2is4
PubMed	17190599
UniProt	P03018\|UVRD_ECOLI DNA helicase II (Gene Name=uvrD)

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