Structure of PDB 2io8 Chain B

Receptor sequence
>2io8B (length=593) Species: 562 (Escherichia coli) [Search protein sequence]
APFGTLLGYAPGGVAIYSSDAVFRSYIDDEYMGHKWQCVEFARRFLFLNY
GVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWD
KGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVEN
GCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQ
FDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELH
LMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAG
AWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELGW
DAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVFAAVPIRTGHPQNEVRL
IDVLLRPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDEL
VKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPK
VDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVK
3D structure
PDB2io8 Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase
ChainB
Resolution2.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.1.78: glutathionylspermidine amidase.
6.3.1.8: glutathionylspermidine synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B E330 N332 E309 N311
BS02 MG B D318 E330 D297 E309
BS03 CYS B S337 C338 E341 R598 I611 S316 C317 E320 R573 I586
BS04 ADP B Y329 E330 K498 A531 K533 R538 G540 Q568 W571 Q582 L603 V604 I605 Y308 E309 K473 A506 K508 R513 G515 Q543 W546 Q557 L578 V579 I580
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008884 glutathionylspermidine amidase activity
GO:0008885 glutathionylspermidine synthase activity
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0008216 spermidine metabolic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:2io8, PDBe:2io8, PDBj:2io8
PDBsum2io8
PubMed17124497
UniProtP0AES0|GSP_ECOLI Bifunctional glutathionylspermidine synthetase/amidase (Gene Name=gss)

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