Structure of PDB 2io7 Chain B

Receptor sequence
>2io7B (length=587) Species: 562 (Escherichia coli) [Search protein sequence]
FGTLLGYAPGGVAIYSAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVF
TDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGE
FKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYT
LKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGK
WLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYL
HATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERG
LKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKH
SRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELGWDAAG
QLIDGEGRLVNCVWKTWAWETAFDQIREAAVPIRTGHPQNEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYA
VKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYI
QVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVK
3D structure
PDB2io7 Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase
ChainB
Resolution2.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.1.78: glutathionylspermidine amidase.
6.3.1.8: glutathionylspermidine synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B E330 N332 E305 N307
BS02 MG B D318 E330 D293 E305
BS03 ANP B R316 D318 Y329 E330 N332 K498 A531 K533 R538 C539 G540 Q568 L570 W571 Q582 L603 V604 I605 R291 D293 Y304 E305 N307 K467 A500 K502 R507 C508 G509 Q537 L539 W540 Q551 L572 V573 I574
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008884 glutathionylspermidine amidase activity
GO:0008885 glutathionylspermidine synthase activity
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0008216 spermidine metabolic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2io7, PDBe:2io7, PDBj:2io7
PDBsum2io7
PubMed17124497
UniProtP0AES0|GSP_ECOLI Bifunctional glutathionylspermidine synthetase/amidase (Gene Name=gss)

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