Structure of PDB 2ihm Chain B

Receptor sequence

>2ihmB (length=336) Species: 10090 (Mus musculus)

SMPAYACQRPSPLTHHNTLLSEALETLAEAAGFEANEGRLLSFSRAASVL
KSLPCPVASLSQLHGLPYFGEHSTRVIQELLEHGTCEEVKQVRCSERYQT
MKLFTQVFGVGVKTANRWYQEGLRTLDELREQPQRLTQQQKAGLQYYQDL
STPVRRADAEALQQLIEAAVRQTLPGATVTLTGGFRRGKLQGHDVDFLIT
HPEEGQEVGLLPKVMSCLQSQGLVLYHQYFERSFCILGLPQPQQAGALPP
CPTWKAVRVDLVVTPSSQFPFALLGWTGSQFFERELRRFSRQEKGLWLNS
HGLFDPEQKRVFHATSEEDVFRLLGLKYLPPEQRNA

3D structure

• PDB: 2ihm Structural insight into the substrate specificity of DNA Polymerase mu.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D330 D332
• Catalytic site (residue number reindexed from 1): D194 D196
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	L177 Q364 F387 E388 R389 R444 R447 R451 L458 N459	L41 Q228 F230 E231 R232 R284 R287 R291 L298 N299
BS02	dna	B	F244 G245 G247 T250 H329 F391 R418 D420 W436	F108 G109 G111 T114 H193 F234 R258 D260 W276
BS03	dna	B	G174 R175 S178 F179 Y204 G206 H208 S209	G38 R39 S42 F43 Y68 G70 H72 S73
BS04	D3T	B	G320 R323 K325 G328 H329 D330 D332 G435 W436 G438 Q440	G184 R187 K189 G192 H193 D194 D196 G275 W276 G278 Q280
BS05	MG	B	D330 D332	D194 D196

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:2ihm, PDBe:2ihm, PDBj:2ihm
• PDBsum: 2ihm
• PubMed: 17159995
• UniProt: Q9JIW4|DPOLM_MOUSE DNA-directed DNA/RNA polymerase mu (Gene Name=Polm)