Structure of PDB 2hmf Chain B

Receptor sequence

>2hmfB (length=464) Species: 2190 (Methanocaldococcus jannaschii)

TTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVE
ISQQALDVRDIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRI
EELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIAL
EGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGY
ITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPK
LSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITND
MEMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVIL
ISQGSSETNISLVVSEEDVDKALKALKREFGDFLNNNLIRDVSVDKDVCV
ISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDL
LNCVRKLHEKFIEK

3D structure

• PDB: 2hmf The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.2.4: aspartate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	B	G8 T230 D231 V232 Y236 T238 D239 P240 R241 A265 K266 V267	G7 T229 D230 V231 Y235 T237 D238 P239 R240 A264 K265 V266
BS02	ASP	B	S40 T46 E130 F193 R207 G208 S210	S39 T45 E129 F192 R206 G207 S209

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004072 aspartate kinase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity

Biological Process

• GO:0008652 amino acid biosynthetic process
• GO:0009088 threonine biosynthetic process
• GO:0009089 lysine biosynthetic process via diaminopimelate
• GO:0009090 homoserine biosynthetic process
• GO:0016310 phosphorylation

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:2hmf, PDBe:2hmf, PDBj:2hmf
• PDBsum: 2hmf
• PubMed: 17012784
• UniProt: Q57991|AK_METJA Probable aspartokinase (Gene Name=MJ0571)