Structure of PDB 2gmj Chain B

Receptor sequence

>2gmjB (length=578) Species: 9823 (Sus scrofa)

PRITTHYTIYPRDQDKRWEGVNMERFAEEADVVIVGAGPAGLSAATRLKQ
LAAQHEKDLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGAPLN
TPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYVVRLGHLVSWMGEQAEA
LGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHA
KVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKWKPG
RVDHTVGWPLDRHTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSPFRE
FQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSP
GFMNVPKIKGTHTAMKSGTLAAESIFNQLTSENLQSKTIGLHVTEYEDNL
KNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTLKHK
GSDSDQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTL
KDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHC
KTCDIKDPSQNINWVVPEGGGGPAYNGM

3D structure

• PDB: 2gmj Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S82 R331 T367 C528 C553 C556 C559
• Catalytic site (residue number reindexed from 1): S76 R325 T361 C522 C547 C550 C553
• Enzyme Commision number: 1.5.5.1: electron-transferring-flavoprotein dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	C528 P529 V532 C553 V554 C556 K557 C559	C522 P523 V526 C547 V548 C550 K551 C553
BS02	FAD	B	G42 P45 A46 E71 K72 H79 S82 G83 A84 C85 A167 E213 G214 Y277 G353 C354 I364 K365 G366 T367	G36 P39 A40 E65 K66 H73 S76 G77 A78 C79 A161 E207 G208 Y271 G347 C348 I358 K359 G360 T361

Gene Ontology

Molecular Function

• GO:0004174 electron-transferring-flavoprotein dehydrogenase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0048039 ubiquinone binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0022900 electron transport chain
• GO:0022904 respiratory electron transport chain

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0016020 membrane

External links

• PDB: RCSB:2gmj, PDBe:2gmj, PDBj:2gmj
• PDBsum: 2gmj
• PubMed: 17050691
• UniProt: P55931|ETFD_PIG Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (Gene Name=ETFDH)