Structure of PDB 2gmh Chain B

Receptor sequence

>2gmhB (length=578) Species: 9823 (Sus scrofa)

PRITTHYTIYPRDQDKRWEGVNMERFAEEADVVIVGAGPAGLSAATRLKQ
LAAQHEKDLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGAPLN
TPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYVVRLGHLVSWMGEQAEA
LGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHA
KVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKWKPG
RVDHTVGWPLDRHTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSPFRE
FQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSP
GFMNVPKIKGTHTAMKSGTLAAESIFNQLTSENLQSKTIGLHVTEYEDNL
KNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTLKHK
GSDSDQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTL
KDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHC
KTCDIKDPSQNINWVVPEGGGGPAYNGM

3D structure

• PDB: 2gmh Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S82 R331 T367 C528 C553 C556 C559
• Catalytic site (residue number reindexed from 1): S76 R325 T361 C522 C547 C550 C553
• Enzyme Commision number: 1.5.5.1: electron-transferring-flavoprotein dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	L504 C528 P529 V532 Y533 C553 V554 C556 K557 C559 W570	L498 C522 P523 V526 Y527 C547 V548 C550 K551 C553 W564
BS02	FAD	B	V41 G42 P45 A46 E71 K72 S82 G83 A84 C85 A167 E213 G214 H218 G353 C354 I364 K365 G366 T367	V35 G36 P39 A40 E65 K66 S76 G77 A78 C79 A161 E207 G208 H212 G347 C348 I358 K359 G360 T361
BS03	UQ5	B	F114 M133 H260 T261 V262 Y271 G272 G273 C425 G434 M435 T438 G439	F108 M127 H254 T255 V256 Y265 G266 G267 C419 G428 M429 T432 G433

Gene Ontology

Molecular Function

• GO:0004174 electron-transferring-flavoprotein dehydrogenase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0048039 ubiquinone binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0022900 electron transport chain
• GO:0022904 respiratory electron transport chain

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0016020 membrane

External links

• PDB: RCSB:2gmh, PDBe:2gmh, PDBj:2gmh
• PDBsum: 2gmh
• PubMed: 17050691
• UniProt: P55931|ETFD_PIG Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (Gene Name=ETFDH)