Structure of PDB 2gih Chain B

Receptor sequence
>2gihB (length=251) Species: 727 (Haemophilus influenzae) [Search protein sequence]
SFIKPIYQDINSILIGQKVKRSGHAAGEPFEKLVYKFLKENLSDLTFKQY
EYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSIENLFEE
KQNDTADILLVKDQFYELLDVKTRNISKSAFAPNIISAYKLAQTCAKMID
NKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYINWAAAMQ
IQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVKPFKKYI
L
3D structure
PDB2gih Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
ChainB
Resolution2.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Q109 N110 D127 T130 K135 A137 F138 A139 P140 N141 S144 K147 A205 M206 Q207 Q102 N103 D120 T123 K128 A130 F131 A132 P133 N134 S137 K140 A198 M199 Q200 PDBbind-CN: Kd=1.03nM
BS02 dna B G92 K93 Q109 F138 Y199 N201 A204 R241 K248 G85 K86 Q102 F131 Y192 N194 A197 R234 K241 PDBbind-CN: Kd=1.03nM
BS03 CA B D114 D127 V128 D107 D120 V121
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2gih, PDBe:2gih, PDBj:2gih
PDBsum2gih
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

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