Structure of PDB 2fb3 Chain B

Receptor sequence

>2fb3B (length=326) Species: 1280 (Staphylococcus aureus)

QIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFD
EMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLTT
NGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQID
YATSIGLNVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDN
GWDFSKVVTKDEMLTMIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLI
TSVSQSFCSTCTRARLSSDGKFYGCLFATVDGFNVKAFIRSGVTDEELKE
QFKALWQIRDDRYSDERTAQTVANRQ

3D structure

• PDB: 2fb3 Binding of 5'-GTP to the C-terminal FeS cluster of the radical S-adenosylmethionine enzyme MoaA provides insights into its mechanism
• Chain: B
• Resolution: 2.349 Å

Enzymatic activity

• Enzyme Commision number: 4.1.99.22: GTP 3',8-cyclase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MET	B	G75 E76 T102 T103 N104 S126	G72 E73 T99 T100 N101 S123
BS02	SF4	B	C24 F26 C28 C31 M32 G75 N104	C21 F23 C25 C28 M29 G72 N101
BS03	SF4	B	C261 C264 C278 L279 F280 R312 D314 Y316 S317	C258 C261 C275 L276 F277 R309 D311 Y313 S314
BS04	POP	B	R71 N124 R192	R68 N121 R189

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005525 GTP binding
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding
• GO:0061798 GTP 3',8'-cyclase activity
• GO:0061799 cyclic pyranopterin monophosphate synthase activity
• GO:1904047 S-adenosyl-L-methionine binding

Biological Process

• GO:0006777 Mo-molybdopterin cofactor biosynthetic process

External links

• PDB: RCSB:2fb3, PDBe:2fb3, PDBj:2fb3
• PDBsum: 2fb3
• PubMed: 16632608
• UniProt: P69848|MOAA_STAA8 GTP 3',8-cyclase (Gene Name=moaA)