Structure of PDB 2f49 Chain B

Receptor sequence
>2f49B (length=337) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
MPKRIVYNISSDFQLKSLLGEGAYGVVCSATHKPTGEIVAIKKIEPFDKP
LFALRTLREIKILKHFKHENIITIFNIQRPDSFENFNEVYIIQELMQTDL
HRVISTQMLSDDHIQYFIYQTLRAVKVLHGSNVIHRDLKPSNLLINSNCD
LKVCDFGLARIIDVEFVATRWYRAPEVMLTSAKYSRAMDVWSCGCILAEL
FLRRPIFPGRDYRHQLLLIFGIIGTPHSDNDLRCIESPRAREYIKSLPMY
PAAPLEKMFPRVNPKGIDLLQRMLVFDPAKRITAKEALEHPYLQTYHDPN
DEPEGEPIPPSFFEFDHYKEALTTKDLKKLIWNEIFS
3D structure
PDB2f49 The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway
ChainB
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D137 K139 N142 D155 T185
Catalytic site (residue number reindexed from 1) D137 K139 N142 D155 T169
Enzyme Commision number 2.7.11.24: mitogen-activated protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B Y24 G25 I44 E45 Y24 G25 I44 E45
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004707 MAP kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0042802 identical protein binding
GO:0106310 protein serine kinase activity
Biological Process
GO:0000749 response to pheromone triggering conjugation with cellular fusion
GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion
GO:0001403 invasive growth in response to glucose limitation
GO:0006468 protein phosphorylation
GO:0010526 retrotransposon silencing
GO:0016310 phosphorylation
GO:0035556 intracellular signal transduction
GO:0043409 negative regulation of MAPK cascade
GO:0046827 positive regulation of protein export from nucleus
GO:0051301 cell division
GO:0071507 pheromone response MAPK cascade
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0010494 cytoplasmic stress granule
GO:0042597 periplasmic space
GO:0043332 mating projection tip

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2f49, PDBe:2f49, PDBj:2f49
PDBsum2f49
PubMed16424299
UniProtP16892|FUS3_YEAST Mitogen-activated protein kinase FUS3 (Gene Name=FUS3)

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