Structure of PDB 2ez4 Chain B

Receptor sequence

>2ez4B (length=585) Species: 1590 (Lactiplantibacillus plantarum) [Search protein sequence]

TNILAGAAVIKVLEAWGVDHLYGIPGGSINSIMDALSAERDRIHYIQVRH
EEVGAMAAAADAKLTGKIGVCFGSAGPGGTHLMNGLYDAREDHVPVLALI
GQFGTTGMNMDTFQEMNENPIYADVADYNVTAVNAATLPHVIDEAIRRAY
AHQGVAVVQIPVDLPWQQIPAEDWYASANSYQTPLLPEPDVQAVTRLTQT
LLAAERPLIYYGIGARKAGKELEQLSKTLKIPLMSTYPAKGIVADRYPAY
LGSANRVAQKPANEALAQADVVLFVGNNYPFAEVSKAFKNTRYFLQIDID
PAKLGKRHKTDIAVLADAQKTLAAILAQVSERESTPWWQANLANVKNWRA
YLASLEDKQEGPLQAYQVLRAVNKIAEPDAIYSIDVGDINLNANRHLKLT
PSNRHITSNLFATMGVGIPGAIAAKLNYPERQVFNLAGDGGASMTMQDLA
TQVQYHLPVINVVFTNCQYGWIKDEQEDTNQNDFIGVEFNDIDFSKIADG
VHMQAFRVNKIEQLPDVFEQAKAIAQHEPVLIDAVITGDRPLPAEKLRLD
SAMSSAADIEAFKQRYEAQDLQPLSTYLKQFGLDD

3D structure

PDB

2ez4 The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography.

Chain

Resolution

2.03 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	I32 G34 G35 S36 I37 E59 S82 F121 Q122 E123 V170 R264 E291 V394 A420 M422 D447 N474 Q476 Y477 W479 I480 E483 G546
Catalytic site (residue number reindexed from 1)	I24 G26 G27 S28 I29 E51 S74 F113 Q114 E115 V162 R256 E283 V386 A412 M414 D439 N466 Q468 Y469 W471 I472 E475 G538
Enzyme Commision number	1.2.3.3: pyruvate oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	MG	B	D447 N474 Q476	D439 N466 Q468
BS02	PO4	B	W479 E483	W471 E475
BS03	TPP	B	D396 A420 M422 G446 D447 G448 G449 N474 Q476 Y477 G478 W479 I480	D388 A412 M414 G438 D439 G440 G441 N466 Q468 Y469 G470 W471 I472
BS04	FAD	B	H101 G220 I221 G222 T244 Y245 P246 A262 N263 R264 V265 G284 N285 N286 P288 F289 D306 I307 K311 D325 A326 N417	H93 G212 I213 G214 T236 Y237 P238 A254 N255 R256 V257 G276 N277 N278 P280 F281 D298 I299 K303 D317 A318 N409

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0016491	oxidoreductase activity
GO:0030976	thiamine pyrophosphate binding
GO:0046872	metal ion binding
GO:0047112	pyruvate oxidase activity

View graph for
Molecular Function

External links

PDB	RCSB:2ez4, PDBe:2ez4, PDBj:2ez4
PDBsum	2ez4
PubMed	16680160
UniProt	P37063\|POXB_LACPL Pyruvate oxidase (Gene Name=pox5)

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