Structure of PDB 2e90 Chain B

Receptor sequence

>2e90B (length=304) Species: 4932 (Saccharomyces cerevisiae)

KNKMEAKIDELINNDPVWSSQNESLISKPYNHILLKKNFRLNLIVQINRV
MNLPKDQLAIVSQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPST
INTANYMYFRAMQLVSQLTTKEPLYHNLITIFNEELINLHRGQGLDIYWR
DFLPEIIPTQEMYLNMVMNKTGGLFRLTLRLMEALSPSHGHSLVPFINLL
GIIYQIRDDYLNLKDFQMGFAEDITEGKLSFPIVHALNFTKTKGQTEQHN
EILRILLLRTSDKDIKLKLIQILEFDTNSLAYTKNFINQLVNMIKNDNEN
KYLP

3D structure

• PDB: 2e90 Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases
• Chain: B
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.-
  2.5.1.1: dimethylallyltranstransferase.
  2.5.1.10: (2E,6E)-farnesyl diphosphate synthase.
  2.5.1.29: geranylgeranyl diphosphate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	D80 D84	D76 D80
BS02	MG	B	D80 D84	D76 D80
BS03	FPP	B	L72 S76 D80 R89 Y112 L143 K174 L178 Q211 D214 K238	L68 S72 D76 R85 Y108 L139 K170 L174 Q205 D208 K228

Gene Ontology

Molecular Function

• GO:0004161 dimethylallyltranstransferase activity
• GO:0004311 farnesyltranstransferase activity
• GO:0004337 geranyltranstransferase activity
• GO:0004659 prenyltransferase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0015031 protein transport
• GO:0016114 terpenoid biosynthetic process
• GO:0033384 geranyl diphosphate biosynthetic process
• GO:0033386 geranylgeranyl diphosphate biosynthetic process
• GO:0045337 farnesyl diphosphate biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion

External links

• PDB: RCSB:2e90, PDBe:2e90, PDBj:2e90
• PDBsum: 2e90
• PubMed: 17535895
• UniProt: Q12051|GGPPS_YEAST Geranylgeranyl pyrophosphate synthase BTS1 (Gene Name=BTS1)