Structure of PDB 2e8u Chain B

Receptor sequence

>2e8uB (length=301) Species: 4932 (Saccharomyces cerevisiae)

KIDELINNDPVWSSQNESLISKPYNHILLKPGKNFRLNLIVQINRVMNLP
KDQLAIVSQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPSTINTA
NYMYFRAMQLVSQLTTKEPLYHNLITIFNEELINLHRGQGLDIYWRDFLP
EIIPTQEMYLNMVMNKTGGLFRLTLRLMEALSPSSHSLVPFINLLGIIYQ
IRDDYLNLKDFQFAEDITEGKLSFPIVHALNFTKTKGQTEQHNEILRILL
LRTSDKDIKLKLIQILEFDTNSLAYTKNFINQLVNMIKNDDELLYIIDHL
S

3D structure

• PDB: 2e8u Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases
• Chain: B
• Resolution: 2.08 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.-
  2.5.1.1: dimethylallyltranstransferase.
  2.5.1.10: (2E,6E)-farnesyl diphosphate synthase.
  2.5.1.29: geranylgeranyl diphosphate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	D80 D84	D72 D76
BS02	MG	B	D80 D84	D72 D76
BS03	IPE	B	G40 K41 R44 H73 R90 Y210	G32 K33 R36 H65 R82 Y199
BS04	IPE	B	D80 R89 K174 D214 K238	D72 R81 K166 D203 K221

Gene Ontology

Molecular Function

• GO:0004161 dimethylallyltranstransferase activity
• GO:0004311 farnesyltranstransferase activity
• GO:0004337 geranyltranstransferase activity
• GO:0004659 prenyltransferase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0015031 protein transport
• GO:0016114 terpenoid biosynthetic process
• GO:0033384 geranyl diphosphate biosynthetic process
• GO:0033386 geranylgeranyl diphosphate biosynthetic process
• GO:0045337 farnesyl diphosphate biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion

External links

• PDB: RCSB:2e8u, PDBe:2e8u, PDBj:2e8u
• PDBsum: 2e8u
• PubMed: 17535895
• UniProt: Q12051|GGPPS_YEAST Geranylgeranyl pyrophosphate synthase BTS1 (Gene Name=BTS1)