Structure of PDB 2dub Chain B

Receptor sequence
>2dubB (length=254) Species: 10116 (Rattus norvegicus) [Search protein sequence]
NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPA
VGAIVLTGGEKAFAAGADIKEMQNRTFQDCYSHWDHITRIKKPVIAAVNG
YALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKS
LAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCAEKIANNSKII
VAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN
FKDH
3D structure
PDB2dub The crystal structure of enoyl-CoA hydratase complexed with octanoyl-CoA reveals the structural adaptations required for binding of a long chain fatty acid-CoA molecule.
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) A98 M103 S113 G141 E144 P163 E164 T169 P171 G172 K257 F267
Catalytic site (residue number reindexed from 1) A67 M72 S82 G105 E108 P127 E128 T133 P135 G136 K221 F231
Enzyme Commision number 4.2.1.17: enoyl-CoA hydratase.
5.3.3.8: Delta(3)-Delta(2)-enoyl-CoA isomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CO8 B K260 F279 K282 K224 F243 K246
BS02 CO8 B K56 A57 L58 A60 A96 G97 A98 D99 I100 K101 G140 G141 E144 P163 E164 G172 A173 K25 A26 L27 A29 A65 G66 A67 D68 I69 K70 G104 G105 E108 P127 E128 G136 A137
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
GO:0004300 enoyl-CoA hydratase activity
GO:0016829 lyase activity
GO:0016836 hydro-lyase activity
GO:0016853 isomerase activity
GO:0043956 3-hydroxypropionyl-CoA dehydratase activity
GO:0120092 crotonyl-CoA hydratase activity
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006635 fatty acid beta-oxidation
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2dub, PDBe:2dub, PDBj:2dub
PDBsum2dub
PubMed9480773
UniProtP14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial (Gene Name=Echs1)

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